TM4 Cell Line

• Catalog number
  EP-CL-0456
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  TM4
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  11 to 13 days
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone(LH). The FSH responsiveness is much reduced compared to primary sertoli cell cultures. Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid.Tested and found negative for ectromelia virus(mousepox).
• Biosafety
  1
• Medium
  DMEM/F12 (PM150312)+5% HS (164215-100)+2.5% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  No
• Effects
  No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
• Receptor_expression
  Follicle stimulating hormone(FSH), expressed; androgen receptor, expressed; progesterone receptor, expressed
• Antigen_expression
  H-Y antigen; Mus musculus, expressed
• Gene_expression
  Retinol Binding Protein, Tissue Plasminogen Activator, Transferrin
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  TM4   Line  
• Gene symbol
  TSPAN16, TSPAN3
• Short name
  TM4 Line
• Species
  Mus musculus, Mouse
• Alternative name
  TM4 cellular Line
• Tissue
  Testis

Gene info

• Identity
  HGNC:30725
• Gene
  TSPAN16
• Long gene name
  tetraspanin 16
• Synonyms gene
  • TM4SF16
• Synonyms gene name
  • transmembrane 4 superfamily member 16
• Synonyms
  • TM4-B
  • TM-8
• GenBank acession
  • BC029908
• Locus
  19p13.2
• Discovery year
  2004-10-19
• Entrez gene record
  26526
• Pubmed identfication
  • 10500248
• RefSeq identity
  • NM_012466
• Classification
  • Tetraspanins
• VEGA ID
  OTTHUMG00000180833

Gene info

• Identity
  HGNC:17752
• Gene
  TSPAN3
• Long gene name
  tetraspanin 3
• Synonyms gene
  • TM4SF8
• Synonyms gene name
  • transmembrane 4 superfamily member 8
• Synonyms
  • TM4-A
  • TSPAN-3
• Locus
  15q24.3
• Discovery year
  2003-07-21
• Entrez gene record
  10099
• RefSeq identity
  • NM_005724
• Classification
  • Tetraspanins
• VEGA ID
  OTTHUMG00000143728

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