TF-1 Cell Line

Abbreviation

TF-1
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

35 years
Morphology

Lymphoblast
Growth_properties

Mixed, Adherent And Suspension
Doubling_time

NA
Background

NA
Biosafety

1
Medium

RPMI-1640 (PM150110)+2ng/mL rhGM-CSF+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

NA
Effects

NA
Receptor_expression

NA
Antigen_expression

NA
Gene_expression

NA
Applications

NA
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.

Gene target

TF-1 Line
Gene symbol

TF, F3, MT-TF, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1
Short name

TF-1 Line
Species

Homo sapiens, Human
Alternative name

TF-1 cellular Line
Tissue

Peripheral blood

Identity

HGNC:7481
Gene

MT-TF
Long gene name

mitochondrially encoded tRNA-Phe (UUU/C)
Synonyms gene
- MTTF
Synonyms gene name
- tRNA phenylalanine
- mitochondrially encoded tRNA phenylalanine
Synonyms
- trnF
Locus

mitochondria
Discovery year

1989-10-11
Entrez gene record

4558
Pubmed identfication
- 7219534
Classification
- Mitochondrially encoded transfer RNAs

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081