SW620 Cell Line

  • Catalog number
    EP-CL-0225
  • Price
    Please ask
  • Size
    1×10⁶ cells/vial
  • Abbreviation
    SW620
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    51 years
  • Morphology
    Epithelial
  • Growth_properties
    Adherent
  • Doubling_time
    NA
  • Background
    A recurrence of the malignancy resulted in a wide-spread metastasis from the colon to an abdominal mass.The established cell line consists mainly of individual small spherical and bipolar cells lacking microvilli.The cells synthesize only small quantities of carcinoembryonic antigen(CEA), and are highly tumorigenic in nude mice.The cells are negative for expression of CSAp(CSAp-) and colon antigen 3, negative.The cells are positive for keratin by immunoperoxidase staining.The line was derived from a metastasis of the same tumor from which the SW480 was derived.There is a G-】 A mutation in codon 273 of the p53 gene resulting in an Arg-】 His substitution.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.N-myc oncogene expression was not detected.
  • Biosafety
    1
  • Medium
    DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture_conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
  • Tumorigenic
    Yes
  • Effects
    Yes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
  • Receptor_expression
    NA
  • Antigen_expression
    Blood Type A; Rh+
  • Gene_expression
    Carcinoembryonic Antigen(Cea) 0.15Ng/10^6 Cells/10 Days; Transforming Growth Factor Alpha; Matrilysin. The Cells Are Negative For Expression Of Csap(Csap-) And Colon Antigen 3, Negative. The Cells Are Positive For Keratin By Immunoperoxidase Staining. The Line Is Positive For Expression Of C-Myc, K-Ras, H-Ras, N-Ras, Myb, Sis And Fos Oncogenes.
  • Applications
    NA
  • Duration
    NA
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
    SW620   Line  
  • Short name
    SW620 Line
  • Species
    Homo sapiens, Human
  • Alternative name
    SW620 cellular Line
  • Tissue
    Colon, derived from metastatic site: lymph node
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