SW579 [SW 579; SW-579] Cell Line

• Catalog number
  EP-CL-0224
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  SW579 [SW 579; SW-579]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  59 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  A recurrence of the malignancy resulted in a wide-spread metastasis from the colon to an abdominal mass. The established cell line consists mainly of individual small spherical and bipolar cells lacking microvilli. The cells synthesize only small quantities of carcinoembryonic antigen(CEA), and are highly tumorigenic in nude mice. The cells are negative for expression of CSAp(CSAp-) and colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line was derived from a metastasis of the same tumor from which the SW480 was derived. There is a G-】 A mutation in codon 273 of the p53 gene resulting in an Arg-】 His substitution. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. N-myc oncogene expression was not detected.
• Biosafety
  1
• Medium
  DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, forms tumors in nude mice.
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  Blood Type O; Rh＋
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  SW579   [SW   579   SW-579]   Line  
• Gene symbol
  MIR579, TUBB, MEX3D, SLC4A1, CDK5RAP3
• Short name
  SW579 [SW 579; SW-579] Line
• Species
  Homo sapiens, Human
• Alternative name
  SW579 [SW 579; SW-579] cellular Line
• Tissue
  Thyroid

Gene info

• Identity
  HGNC:32835
• Gene
  MIR579
• Long gene name
  microRNA 579
• Synonyms gene
  • MIRN579
• Synonyms
  • hsa-mir-579
• Locus
  5p13.3
• Discovery year
  2006-05-16
• Entrez gene record
  693164
• RefSeq identity
  • NR_030305
• Classification
  • MicroRNA MIR548 family

Gene info

• Identity
  HGNC:20778
• Gene
  TUBB
• Long gene name
  tubulin beta class I
• Synonyms gene name
  • tubulin, beta polypeptide
  • tubulin, beta
  • tubulin, beta class I
• Synonyms
  • OK/SW-cl.56
  • MGC16435
  • M40
  • Tubb5
• Synonyms name
  • class I beta-tubulin
  • beta1-tubulin
• GenBank acession
  • AB062393
• Locus
  6p21.33
• Discovery year
  2004-11-22
• Entrez gene record
  203068
• Pubmed identfication
  • 11504633
  • 8270253
• RefSeq identity
  • NM_178014
• Classification
  • Tubulins
• VEGA ID
  OTTHUMG00000031059

Gene info

• Identity
  HGNC:16734
• Gene
  MEX3D
• Long gene name
  mex-3 RNA binding family member D
• Synonyms gene
  • RKHD1
• Synonyms gene name
  • ring finger and KH domain containing 1
  • mex-3 homolog D (C. elegans)
• Synonyms
  • Tino
  • KIAA2031
  • OK/SW-cl.4
  • RNF193
• Synonyms name
  • bcl-2 ARE RNA binding protein
• GenBank acession
  • AB107353
• Locus
  19p13.3
• Discovery year
  2004-03-17
• Entrez gene record
  399664
• Pubmed identfication
  • 17267406
• RefSeq identity
  • NM_203304
• Classification
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000150369

Gene info

• Identity
  HGNC:11027
• Gene
  SLC4A1
• Long gene name
  solute carrier family 4 member 1 (Diego blood group)
• Synonyms gene
  • EPB3
  • AE1
  • DI
  • WD
• Synonyms gene name
  • Waldner blood group
  • erythrocyte membrane protein band 3
  • Diego blood group
  • solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
  • solute carrier family 4 (anion exchanger), member 1
  • solute carrier family 4 (anion exchanger), member 1 (Diego blood group)
• Synonyms
  • RTA1A
  • CD233
  • FR
  • SW
  • WR
• Synonyms name
  • Froese blood group
  • Swann blood group
  • Wright blood group
• Locus
  17q21.31
• Discovery year
  1988-04-20
• Entrez gene record
  6521
• Pubmed identfication
  • 8434259
• RefSeq identity
  • NM_000342
• Classification
  • Blood group antigens
  • Solute carriers
  • CD molecules
• VEGA ID
  OTTHUMG00000156843
• Locus Specific Databases
  • Blood Group Antigen Mutation Database
  • LRG_803

Gene info

• Identity
  HGNC:18673
• Gene
  CDK5RAP3
• Long gene name
  CDK5 regulatory subunit associated protein 3
• Synonyms
  • MST016
  • FLJ13660
  • C53
  • IC53
  • HSF-27
  • OK/SW-cl.114
  • LZAP
• Synonyms name
  • ischemic heart CDK5 activator-binding protein C53
  • LXXLL/leucine-zipper-containing ARFbinding protein
• GenBank acession
  • AF110322
• Locus
  17q21.32
• Discovery year
  2002-07-22
• Entrez gene record
  80279
• Pubmed identfication
  • 10721722
• RefSeq identity
  • NM_176096
• VEGA ID
  OTTHUMG00000178682

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