SW480 [SW-480] Cell Line

• Catalog number
  EP-CL-0223
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  SW480 [SW-480]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  50 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  A cell line established from a lymph node metastasis taken from the same patient one year later is available.The line is negative for CSAp(CSAp-) and colon antigen 3.The cells are positive for keratin by immunoperoxidase staining.There is a G-】 A mutation in codon 273 of the p53 gene resulting in an Arg-】 His substitution and a C-】 T mutation in codon 309 resulting in a Pro-】 Ser substitution.The cells express elevated levels of p53 protein.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.N-myc oncogene expression was not detected.Matrilysin, a metalloproteinase associated with tumor invasiveness, is not expressed.The cells have been reported to produce GM-CSF.
• Biosafety
  1
• Medium
  DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes, Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
• Effects
  Yes, in nude mice.
• Receptor_expression
  Epidermal growth factor(EGF)
• Antigen_expression
  NA
• Gene_expression
  Carcinoembryonic Antigen(Cea) 0.7Ng/10^6 Cells/10 Days; Keratin; Transforming Growth Factor Beta, Myc＋; Myb＋; Ras＋; Fos＋; Sis＋; P53＋; Abl -; Ros -; Src -, Hla A2, B8, B17; Blood Type A; Rh＋, The Cells Are Positive For Keratin By Immunoperoxidase Staining., The Line Is Positive For Expression Of C-Myc, K-Ras, H-Ras, N-Ras, Myb, Sis And Fos Oncogenes.
• Applications
  This cell line is a suitable transfection host. This line has a mutation in codon 12 of the ras proto-oncogene, and can be used as a positive control for PCR assays of mutation in this codon.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  SW480   [SW-480]   Line  
• Gene symbol
  TUBB, MEX3D, SLC4A1, CDK5RAP3
• Short name
  SW480 [SW-480] Line
• Species
  Homo sapiens, Human
• Alternative name
  SW480 [SW-480] cellular Line
• Tissue
  Colon

Gene info

• Identity
  HGNC:20778
• Gene
  TUBB
• Long gene name
  tubulin beta class I
• Synonyms gene name
  • tubulin, beta polypeptide
  • tubulin, beta
  • tubulin, beta class I
• Synonyms
  • OK/SW-cl.56
  • MGC16435
  • M40
  • Tubb5
• Synonyms name
  • class I beta-tubulin
  • beta1-tubulin
• GenBank acession
  • AB062393
• Locus
  6p21.33
• Discovery year
  2004-11-22
• Entrez gene record
  203068
• Pubmed identfication
  • 11504633
  • 8270253
• RefSeq identity
  • NM_178014
• Classification
  • Tubulins
• VEGA ID
  OTTHUMG00000031059

Gene info

• Identity
  HGNC:16734
• Gene
  MEX3D
• Long gene name
  mex-3 RNA binding family member D
• Synonyms gene
  • RKHD1
• Synonyms gene name
  • ring finger and KH domain containing 1
  • mex-3 homolog D (C. elegans)
• Synonyms
  • Tino
  • KIAA2031
  • OK/SW-cl.4
  • RNF193
• Synonyms name
  • bcl-2 ARE RNA binding protein
• GenBank acession
  • AB107353
• Locus
  19p13.3
• Discovery year
  2004-03-17
• Entrez gene record
  399664
• Pubmed identfication
  • 17267406
• RefSeq identity
  • NM_203304
• Classification
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000150369

Gene info

• Identity
  HGNC:11027
• Gene
  SLC4A1
• Long gene name
  solute carrier family 4 member 1 (Diego blood group)
• Synonyms gene
  • EPB3
  • AE1
  • DI
  • WD
• Synonyms gene name
  • Waldner blood group
  • erythrocyte membrane protein band 3
  • Diego blood group
  • solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
  • solute carrier family 4 (anion exchanger), member 1
  • solute carrier family 4 (anion exchanger), member 1 (Diego blood group)
• Synonyms
  • RTA1A
  • CD233
  • FR
  • SW
  • WR
• Synonyms name
  • Froese blood group
  • Swann blood group
  • Wright blood group
• Locus
  17q21.31
• Discovery year
  1988-04-20
• Entrez gene record
  6521
• Pubmed identfication
  • 8434259
• RefSeq identity
  • NM_000342
• Classification
  • Blood group antigens
  • Solute carriers
  • CD molecules
• VEGA ID
  OTTHUMG00000156843
• Locus Specific Databases
  • Blood Group Antigen Mutation Database
  • LRG_803

Gene info

• Identity
  HGNC:18673
• Gene
  CDK5RAP3
• Long gene name
  CDK5 regulatory subunit associated protein 3
• Synonyms
  • MST016
  • FLJ13660
  • C53
  • IC53
  • HSF-27
  • OK/SW-cl.114
  • LZAP
• Synonyms name
  • ischemic heart CDK5 activator-binding protein C53
  • LXXLL/leucine-zipper-containing ARFbinding protein
• GenBank acession
  • AF110322
• Locus
  17q21.32
• Discovery year
  2002-07-22
• Entrez gene record
  80279
• Pubmed identfication
  • 10721722
• RefSeq identity
  • NM_176096
• VEGA ID
  OTTHUMG00000178682

Similar products