SPC-A-1 Cell Line
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Catalog numberEP-CL-0218
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationSPC-A-1
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeNA
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundNA
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Biosafety1
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MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNA
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EffectsNA
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolIGHV5-10-1, UCN3, NR1H3, SPI1, H2AC8, HLA-A, ATP2C1, F11R, CIDEA, RPA1
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Short nameSPC-A-1 Line
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SpeciesHomo sapiens, Human
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Alternative nameSPC-A-1 cellular Line
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TissueLung
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-10-1
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameurocortin 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear receptor subfamily 1 group H member 3
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Synonyms gene name
- nuclear receptor subfamily 1, group H, member 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-09-17
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Entrez gene record
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Pubmed identfication
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Classification
- Nuclear receptor subfamily 1 group H
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSpi-1 proto-oncogene
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Synonyms gene name
- spleen focus forming virus (SFFV) proviral integration oncogene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-07-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2A clustered histone 8
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Synonyms gene
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Synonyms gene name
- H2A histone family, member A
- histone 1, H2ae
- histone cluster 1, H2ae
- histone cluster 1 H2A family member e
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-08-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H2A histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, A
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameATPase secretory pathway Ca2+ transporting 1
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Synonyms gene
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Synonyms gene name
- benign chronic pemphigus (Hailey-Hailey disease)
- ATPase, Ca++ transporting, type 2C, member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-09-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ATPases Ca2+ transporting
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameF11 receptor
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Synonyms gene
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Synonyms gene name
- junctional adhesion molecule 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-04-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ig-like cell adhesion molecule family
- V-set domain containing
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell death inducing DFFA like effector a
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Synonyms gene name
- cell death-inducing DFFA-like effector a
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereplication protein A1
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Synonyms gene name
- replication protein A1 (70kD)
- replication protein A1, 70kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nucleotide excision repair
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VEGA ID