SK-OV-3 Cell Line

• Catalog number
  EP-CL-0215
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  SK-OV-3
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  64 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis-platinum and adriamycin.
• Biosafety
  1
• Medium
  McCoy's 5A (PM150710)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, in nude mice forms moderately well differentiated adenocarcinoma consistent with ovarian primary.
• Receptor_expression
  NA
• Antigen_expression
  Blood Type B; Rh＋
• Gene_expression
  Blood Type B; Rh＋
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  SK-OV-3   Line  
• Gene symbol
  TMOD4, DKK1, MIR1302-3, MIR509-3, MIR7-3, PIRC13, PIRC11, PIRC12
• Short name
  SK-OV-3 Line
• Species
  Homo sapiens, Human
• Alternative name
  SK-OV-3 cellular Line
• Tissue
  Ovary: ascites

Gene info

• Identity
  HGNC:11874
• Gene
  TMOD4
• Long gene name
  tropomodulin 4
• Synonyms gene name
  • tropomodulin 4 (muscle)
• Synonyms
  • Sk-Tmod
• Synonyms name
  • actin-capping protein
  • skeletal tropomodulin
• GenBank acession
  • AF177173
• Locus
  1q21.3
• Discovery year
  2000-02-16
• Entrez gene record
  29765
• Pubmed identfication
  • 10662549
  • 10497209
• Classification
  • Tropomodulins
• VEGA ID
  OTTHUMG00000012350

Gene info

• Identity
  HGNC:2891
• Gene
  DKK1
• Long gene name
  dickkopf WNT signaling pathway inhibitor 1
• Synonyms gene name
  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf 1 homolog (Xenopus laevis)
• Synonyms
  • SK
  • DKK-1
• Locus
  10q21.1
• Discovery year
  2000-09-01
• Entrez gene record
  22943
• RefSeq identity
  • NM_012242
• VEGA ID
  OTTHUMG00000018247

Gene info

• Identity
  HGNC:35295
• Gene
  MIR1302-3
• Long gene name
  microRNA 1302-3
• Synonyms gene
  • MIRN1302-3
• Synonyms
  • hsa-mir-1302-3
• Locus
  2q14.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302128
• RefSeq identity
  • NR_031632
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:33675
• Gene
  MIR509-3
• Long gene name
  microRNA 509-3
• Synonyms gene
  • MIRN509-3
• Synonyms
  • hsa-mir-509-3
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126337
• RefSeq identity
  • NR_030629
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:31640
• Gene
  MIR7-3
• Long gene name
  microRNA 7-3
• Synonyms gene
  • MIRN7-3
• Synonyms
  • hsa-mir-7-3
• Locus
  19p13.3
• Discovery year
  2004-04-23
• Entrez gene record
  407045
• RefSeq identity
  • NR_029607
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:37517
• Gene
  PIRC13
• Long gene name
  piwi-interacting RNA cluster 13
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313925
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37515
• Gene
  PIRC11
• Long gene name
  piwi-interacting RNA cluster 11
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313897
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37516
• Gene
  PIRC12
• Long gene name
  piwi-interacting RNA cluster 12
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313808
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

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