SK-N-SH Cell Line
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Catalog numberEP-CL-0214
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationSK-N-SH
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age4 years
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundNA
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Biosafety1
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MediumMEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNA
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EffectsNA
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Receptor_expressionNA
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Antigen_expressionBlood Type A; Rh+
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Gene_expressionPlasminogen Activator, Shows Increased Expression Of M-Csf After Treatment With Amyloid-Beta Peptide.
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolTMOD4, GH1, DKK1, POLM, NRAS, SDC3, PIGN, WASL, MAP6, HLA-N
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Short nameSK-N-SH Line
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SpeciesHomo sapiens, Human
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Alternative nameSK-N-SH cellular Line
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TissueBrain, derived from metastatic site: bone marrow
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Gene info
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Identity
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Gene
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Long gene nametropomodulin 4
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Synonyms gene name
- tropomodulin 4 (muscle)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-02-16
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Entrez gene record
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Pubmed identfication
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Classification
- Tropomodulins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegrowth hormone 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Growth hormone family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namedickkopf WNT signaling pathway inhibitor 1
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Synonyms gene name
- dickkopf (Xenopus laevis) homolog 1
- dickkopf 1 homolog (Xenopus laevis)
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Synonyms
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Locus
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Discovery year2000-09-01
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDNA polymerase mu
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Synonyms gene name
- polymerase (DNA directed), mu
- polymerase (DNA) mu
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DNA polymerases
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNRAS proto-oncogene, GTPase
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Synonyms gene name
- neuroblastoma RAS viral (v-ras) oncogene homolog
- neuroblastoma RAS viral oncogene homolog
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RAS type GTPase family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesyndecan 3
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Synonyms gene name
- syndecan 3 (N-syndecan)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-05-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Syndecans
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namephosphatidylinositol glycan anchor biosynthesis class N
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Synonyms gene name
- phosphatidylinositol glycan, class N
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Phosphatidylinositol glycan anchor biosynthesis
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameWASP like actin nucleation promoting factor
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Synonyms gene name
- Wiskott-Aldrich syndrome-like
- Wiskott-Aldrich syndrome like
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-10-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Wiskott-Aldrich Syndrome protein family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrotubule associated protein 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-02-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, N (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-10-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases