SK-MES-1 Cell Line

  • Catalog number
    EP-CL-0213
  • Price
    Please ask
  • Size
    1×10⁶ cells/vial
  • Abbreviation
    SK-MES-1
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    65 years adult
  • Morphology
    Epithelial
  • Growth_properties
    Adherent
  • Doubling_time
    NA
  • Background
    NA
  • Biosafety
    1
  • Medium
    MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture_conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
  • Tumorigenic
    NA
  • Effects
    NA
  • Receptor_expression
    NA
  • Antigen_expression
    Blood Type O; Rh+; HLA A3, Aw30, B7, B27
  • Gene_expression
    NA
  • Applications
    NA
  • Duration
    NA
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Buiffer
    MES buffers are Morpholine Ethane Sulphonic Acid or CAS 4432-31 buffers with compound 2-(N-morpholino)ethane sulfonic acid. Its chemical structure contains a morpholine ring. It has a molecular weight of 195.2 g/mol and the chemical formula is C6H13NO4S. Synonyms include 2-morpholinoethanesulfonic acid; 2-(4-morpholino)ethane sulfonic acid; 2-(N-morpholino)ethane sulfonic acid; 2-(4-morpholino)ethane sulfonic acid; MES; MES hydrate; and morpholine-4-ethanesulfonic acid hydrate. MOPS is a similar pH buffering compound which contains a propane sulfonic moiety instead of an ethane sulfonic one.
  • Gene target
    Line  
  • Short name
    SK- -1 Line
  • Species
    Homo sapiens, Human
  • Alternative name
    SK-MES-1 cellular Line
  • Tissue
    Lung, derived from metastatic site: pleural effusion
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