Sf9 Cell Line

• Catalog number
  EP-CL-0205
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  Sf9
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  Pupa
• Morphology
  Round and epithelial
• Growth_properties
  Mixed, Adherent And Suspension
• Doubling_time
  ~50 hours
• Background
  It is important to use the medium described below.
• Biosafety
  1
• Medium
  TNM-FH (PM152010)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air Temperature: 27℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Sf9   Line  
• Short name
  Sf9 Line
• Species
  Spodoptera frugiperda, Fall armyworm
• Alternative name
  Sf9 cellular Line
• Tissue
  Ovary

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