S-180 Cell Line

Abbreviation

S-180
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

NA
Morphology

Rounded
Growth_properties

Mixed, Adherent And Suspension
Doubling_time

NA
Background

Tested and found negative for ectromelia virus(mousepox).
Biosafety

1
Medium

DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

NA
Effects

NA
Receptor_expression

NA
Antigen_expression

NA
Gene_expression

NA
Applications

NA
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.

Gene target

S-180 Line
Gene symbol

LRRC7, B3GAT2, HLA-S, CTSL3P, STRN3, CENPS, MRPS7, H2BC19P, DIABLO, MAG
Short name

S-180 Line
Species

Mus musculus, Mouse
Alternative name

S-180 cellular Line
Tissue

Sarcoma, derived from metastatic site: ascites

Identity

HGNC:922
Gene

B3GAT2
Long gene name

beta-1,3-glucuronyltransferase 2
Synonyms
- GlcAT-S
Synonyms name
- glucuronosyltransferase S
- galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2
GenBank acession
- AB075843
Locus

6q13
Discovery year

2000-01-07
Entrez gene record

135152
Pubmed identfication
- 12383500
RefSeq identity
- NM_080742
Classification
- Beta-1,3-glucuronyltransferases
VEGA ID

OTTHUMG00000014997

Identity

HGNC:15720
Gene

STRN3
Long gene name

striatin 3
Synonyms gene name
- striatin, calmodulin binding protein 3
Synonyms
- SG2NA
- PPP2R6B
- S/G2NA
Synonyms name
- cell cycle S/G2 nuclear autoantigen
- protein phosphatase 2 regulatory subunit B'''beta
Locus

14q12
Discovery year

2002-07-22
Entrez gene record

29966
Pubmed identfication
- 7864889
- 10681496
RefSeq identity
- NM_014574
Classification
- WD repeat domain containing
- STRIPAK complex
- Protein phosphatase 2 regulatory subunits
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000140201

Identity

HGNC:23163
Gene

CENPS
Long gene name

centromere protein S
Synonyms gene
- APITD1
- MHF1
Synonyms gene name
- apoptosis-inducing, TAF9-like domain 1
- FANCM associated histone fold protein 1
Synonyms
- CENP-S
- FAAP16
GenBank acession
- BC029430
Locus

1p36.22
Discovery year

2003-09-25
Entrez gene record

378708
Pubmed identfication
RefSeq identity
- NM_199294
Classification
- Constitutive centromere associated network
VEGA ID

OTTHUMG00000059085

Identity

HGNC:20517
Gene

H2BC19P
Long gene name

H2B clustered histone 19, pseudogene
Synonyms gene
- H2BFO
- HIST2H2BD
Synonyms gene name
- histone 2, H2bd
- H2B histone family, member O
- histone cluster 2, H2bd (pseudogene)
- histone cluster 2 H2B family member d (pseudogene)
Synonyms
- H2B/s
- H2B/o
GenBank acession
- AY131978
Locus

1q21.2
Discovery year

2003-02-20
Entrez gene record

337874
Pubmed identfication
- 12408966
- 15527963
RefSeq identity
- NG_001226
Classification
- H2B histones
VEGA ID

OTTHUMG00000035827

Identity

HGNC:6783
Gene

MAG
Long gene name

myelin associated glycoprotein
Synonyms gene
- GMA
Synonyms
- SIGLEC4A
- SIGLEC-4A
- S-MAG
Synonyms name
- sialic acid binding Ig-like lectin 4A
GenBank acession
- M29273
Locus

19q13.1
Discovery year

2001-06-22
Entrez gene record

4099
Pubmed identfication
- 2476987
- 8432525
RefSeq identity
- NM_080600
Classification
- C2-set domain containing
- I-set domain containing
- Sialic acid binding Ig like lectins
VEGA ID

OTTHUMG00000183296