RH-35 Cell Line
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Catalog numberEP-CL-0195
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationRH-35
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeNA
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundNA
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Biosafety1
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MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNA
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EffectsNA
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolIGLV7-35, SNORD115-35, IGKV1-35, IGHV3-35, IGKV1D-35, RNU6-35P, RHCE, PIAS1, DDX21, PNPT1
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Short nameRH-35 Line
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SpeciesRattus norvegicus, Rat
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Alternative nameRH-35 cellular Line
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TissueLiver
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 7-35 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 7-35
- immunoglobulin lambda variable 7-35 pseudogene
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-35
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-35 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1-35
- immunoglobulin kappa variable 1-35 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-35 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 3-35
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-35 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1D-35
- immunoglobulin kappa variable 1D-35 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 35, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 35
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameRh blood group CcEe antigens
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Synonyms gene
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Synonyms gene name
- Rhesus blood group, CcEe antigens
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-10-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Blood group antigens
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameprotein inhibitor of activated STAT 1
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Synonyms gene
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Synonyms gene name
- DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-11-06
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Entrez gene record
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Pubmed identfication
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Classification
- Zinc fingers MIZ-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDExD-box helicase 21
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Synonyms gene name
- DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21
- DEAD-box helicase 21
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-12-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DEAD-box helicases
- B-WICH chromatin-remodelling complex subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepolyribonucleotide nucleotidyltransferase 1
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Synonyms gene
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Synonyms gene name
- deafness, autosomal recessive 70
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-09-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID