Recombinant Rat UCHL1 / PGP9.5 Protein (His tag) 20µg

#

• Catalog number
  PKSR030367-20µg
• Price:

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• Size
  20µg

# #

• Synonym
  UCHL1
• Activity
  Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/μg.
• Sequence
  Glu 2-Ala 223
• Fusion tag
  N-His
• Accession
  Q00981
• ExpressedHost
  E. coli
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 97 % as determined by SDS-PAGE
• Endotoxin
  Please contact us for more information.
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  26.2 kDa
• AP Mol Mass
  28 KDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.
• Technical Data
• Conjugation
  histidine
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

#

• Gene target
  UCHL1   PGP9   Protein   His   tag   20µg  
• Gene symbol
  UCHL1-DT, UCHL1
• Short name
  Recombinant UCHL1 / PGP9 5 Protein (His tag) 20µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  Rat
• Label
  His
• Species
  Rat, Rats
• Alternative name
  Rec. Rat UCHL1 / PGP9.5 Protein (histidine detection labelled) 20µg
• Alternative technique
  rec

Gene info

• Identity
  HGNC:40600
• Gene
  UCHL1-DT
• Long gene name
  UCHL1 divergent transcript
• Synonyms gene
  • UCHL1-AS1
• Synonyms gene name
  • UCHL1 antisense RNA 1 (head to head)
• GenBank acession
  • DB169394
  • CN277289
• Locus
  4p13
• Discovery year
  2012-12-01
• Entrez gene record
  101410542
• RefSeq identity
  • NR_102709
• Classification
  • Divergent transcripts
• VEGA ID
  OTTHUMG00000160405

Gene info

• Identity
  HGNC:12513
• Gene
  UCHL1
• Long gene name
  ubiquitin C-terminal hydrolase L1
• Synonyms gene
  • PARK5
• Synonyms gene name
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Synonyms
  • PGP9.5
  • Uch-L1
• Synonyms name
  • ubiquitin thiolesterase
• GenBank acession
  • BC000332
• Locus
  4p13
• Discovery year
  1991-07-15
• Entrez gene record
  7345
• Pubmed identfication
  • 1840236
• RefSeq identity
  • NM_004181
• Classification
  • Ubiquitin C-terminal hydrolases
• VEGA ID
  OTTHUMG00000099377

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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