Recombinant Rat CD73 / NT5E Protein (His tag) 20µg

#

• Catalog number
  PKSR030221-20µg
• Price:

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• Size
  20µg

# #

• Synonym
  NT5E
• Activity
  Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).The specific activity is >15,000 pmol/min/µg.
• Sequence
  Met1-Lys549
• Fusion tag
  C-His
• Accession
  Q66HL0
• ExpressedHost
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 90 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  59.4 kDa
• AP Mol Mass
  59 KDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
• Technical Data
• Conjugation
  histidine
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

#

• Gene target
  CD73   NT5E   Protein   His   tag   20µg  
• Gene symbol
  NT5E
• Short name
  Recombinant CD73 / NT5E Protein (His tag) 20µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  Rat
• Label
  His
• Species
  Rat, Rats
• Alternative name
  Rec. Rat CD73 / 5'-nucleotidase, ecto (CD73) Protein (histidine detection labelled) 20µg
• Alternative technique
  rec
• Alternative to gene target
  5'-nucleotidase, ecto (CD73), CALJA and CD73 and E5NT and eN and eNT and NT and NT5 and NTE, NT5E and IDBG-93979 and ENSG00000135318 and 4907, hydrolase activity, Plasma membranes, Nt5e and IDBG-190005 and ENSMUSG00000032420 and 23959

Gene info

• Identity
  HGNC:8021
• Gene
  NT5E
• Long gene name
  5'-nucleotidase ecto
• Synonyms gene
  • NT5
• Synonyms gene name
  • 5' nucleotidase (CD73)
  • 5'-nucleotidase, ecto (CD73)
• Synonyms
  • CD73
  • eN
  • eNT
  • CALJA
• GenBank acession
  • X55740
• Locus
  6q14.3
• Discovery year
  1989-02-23
• Entrez gene record
  4907
• Classification
  • CD molecules
  • 5'-nucleotidases
• VEGA ID
  OTTHUMG00000015139

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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