Recombinant Human VLDLR / VLDL Receptor Protein (His tag)
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Catalog numberPKSH031275-50µg
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PricePlease ask
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Size50µg
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SynonymCAMRQ1;CARMQ1;CHRMQ1;VLDLRCH
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ActivityMeasured by its binding ability in a functional ELISA.Immobilized human VLDLR-His at 10μg/mL (100μL/well) can bind biotinylated human LRPAP1-His, the EC50 of biotinylated human LRPAP1-His is 0.05-0.2 μg/mL.
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SequenceMet 1-Ser 797
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Fusion tagC-His
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AccessionNP_003374.3
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Expressed HostHEK293 Cells
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ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity>95 % as determined by SDS-PAGE
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Endotoxin<1.0 EU per µg as determined by the LAL method
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass86 kDa
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AP Mol Mass150 & 180 kDa
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FormulationLyophilized from sterile PBS, pH 7.4
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BackgroundThe very low density lipoprotein receptor, known as VLDLR, is a single-pass type 1 integral membrance protein and a member of the LDL receptor family. This receptor family includes LDL receptor, LRP, megalin, VLDLR and ApoER2, and is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR contains 3 EGF-like domains, 8 LDL-receptor class A domains, as well as 6 LDL-receptor class B repeats, and is abundant in heart, skeletal muscle, also ovary and kidney, but not in liver. VLDLR binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. VLDLR mediates the phosphorylation of mDab1 (mammalian disabled protein) via binding to Reelin, and induces the modulation of Tau phosphorylation. This pathway regulates the migration of neurons along the radial glial fiber network during brain development. Defects of VLDLR may be the cause of VLDLR-associated cerebellar hypoplasia (VLDLRCH), a syndrome characterized by moderate-to-profound mental retardation, delayed ambulation, and predominantly truncal ataxia.
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Conjugationhistidine
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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DescriptionThe receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolVLDLR-AS1, VLDLR
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Short nameRecombinant VLDLR / VLDL Receptor Protein (His tag)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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LabelHis
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SpeciesHuman, Humans
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Alternative nameRec. H. sapiens very low density lipoprotein receptor / VLDL Receptor Protein (histidine detection labelled)
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Alternative techniquerec
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Alternative to gene targetvery low density lipoprotein receptor, CARMQ1 and CHRMQ1 and VLDLRCH, VLDLR and IDBG-44752 and ENSG00000147852 and 7436, calcium-dependent protein binding, Extracellular, Vldlr and IDBG-155959 and ENSMUSG00000024924 and 22359, VLDLR and IDBG-631615 and ENSBTAG00000018517 and 282123
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Gene info
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Identity
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Gene
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Long gene nameVLDLR antisense RNA 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2014-01-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namevery low density lipoprotein receptor
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Synonyms
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Locus
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Discovery year1993-09-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Low density lipoprotein receptors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data