Recombinant Human E3 Ubiquitin-Protein Ligase CHIP/CHIP Protein 50µg

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• Catalog number
  PKSH032367-50µg
• Price:

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• Size
  50µg

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• Synonym
  E3 Ubiquitin-Protein Ligase CHIP; Antigen NY-CO-7; CLL-Associated Antigen KW-8; Carboxy Terminus of Hsp70-Interacting Protein; STIP1 Homology and U Box-Containing Protein 1; STUB1; CHIP
• Activity
  NA
• Sequence
  Met1-Tyr303
• Fusion tag
  NA
• Accession
  Q9UNE7
• Expressed Host
  E.coli
• Shipping
  The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
• Purity
  ＞90% as determined by reducing SDS-PAGE.
• Endotoxin
  ＜1.0 EU per µg as determined by LAL test.
• Stability and Storage
  Store at < -20℃, stable for 6 monthshs after receipt.Please minimize freeze-thaw cycles
• Mol Mass
  34.9 kDa
• AP Mol Mass
  33 kDa
• Formulation
  Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Reconstitution
  Please refer to it for detailed information.
• Background
  E3 Ubiquitin-Protein Ligase CHIP is a cytoplasmic protein. CHIP is highly expressed in skeletal muscle, heart, pancreas, brain and placenta. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain; lead to in client substrate ubiquitylation and degradation by the proteasome. CHIP targets misfolded chaperone substrates towards proteasomal degradation. CHIP mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. CHIP plays a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. It also may regulate the receptor stability and activity through proteasomal degradation.
• Product URL
• PDF URL
• Properties
  Precipitation of Chromatins is a pull down technique of native DNA sequences by specific binding proteins on which on the other side of the protein the antibody can bind. Lots of antibodies are not suited for Chip analysis pulldown because they bind on the binding site of the DNA. The Elabscience antibody is proven to not bind on the DNA binding site and is suitable for ChiP precipitation supplied in 1 vials. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

#

• Gene target
  Ubiquitin-Protein   Ligase   Protein   50µg  
• Short name
  Recombinant E3 Ubiquitin-Protein Ligase CHIP/CHIP Protein 50µg
• Technique
  Recombinant, ChiP, Chromatine immuno Precipitationg antibody, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens E3 Ubiquitin-Protein Ligase CHIP/CHIP Protein 50µg
• Alternative technique
  rec, epigenetics

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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