Recombinant Human Bruton Tyrosine Kinase / BTK Kinase Protein (His tag) 50µg

• Catalog number
  PKSH030407-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  AGMX1;AT;ATK;BPK;IMD1;PSCTK1;XLA
• Activity
  The specific activity was determined to be 115 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.2. Measured by its ability to bind human BLNK in a functional ELISA.
• Sequence
  Met 1-Ser 659
• Fusion tag
  C-His
• Accession
  NP_000052.1
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
• Purity
  ＞85 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  77.8 kDa
• AP Mol Mass
  77.8 kDa
• Formulation
  Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0
• Reconstitution
  Please refer to it for detailed information.
• Background
  Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Bruton   Tyrosine   Kinase   BTK   Kinase   Protein   His   tag   50µg  
• Gene symbol
  BTK
• Short name
  Recombinant Bruton Tyrosine Kinase / BTK Kinase Protein (His tag) 50µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Bruton Tyrosine phosphorylation catalyst / Bruton agammaglobulinemia tyrosine kinase phosphorylation catalyst Protein (histidine detection labelled) 50µg
• Alternative technique
  rec
• Alternative to gene target
  Bruton agammaglobulinemia tyrosine kinase, AGMX1 and AT and ATK and BPK and IMD1 and PSCTK1 and XLA, BTK and IDBG-79651 and ENSG00000010671 and 695, phosphatidylinositol-3, nuclei, Btk and IDBG-171427 and ENSMUSG00000031264 and 12229, BTK and IDBG-633447 and ENSBTAG00000019250 and 533459

Gene info

• Identity
  HGNC:1133
• Gene
  BTK
• Long gene name
  Bruton tyrosine kinase
• Synonyms gene
  • AGMX1
  • IMD1
• Synonyms gene name
  • Bruton agammaglobulinemia tyrosine kinase
• Synonyms
  • ATK
  • XLA
  • PSCTK1
• Synonyms name
  • Bruton's tyrosine kinase
• GenBank acession
  • AK057105
• Locus
  Xq22.1
• Discovery year
  1986-01-01
• Entrez gene record
  695
• Pubmed identfication
  • 8380905
• RefSeq identity
  • NM_000061
• Classification
  • Tec family tyrosine kinases
  • SH2 domain containing
  • Pleckstrin homology domain containing
• VEGA ID
  OTTHUMG00000022022
• Locus Specific Databases
  • BTK base: Mutation registry for X-linked agammaglobulinemia
  • Global Variome shared LOVD
  • LRG_128

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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