Rat TNF-β(Tumor Necrosis Factor Beta) EIA Kit
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Catalog number
E-EL-R1037-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Rat
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-β. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNF-β. You can calculate the concentration of Rat TNF-β in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
18.75pg/mL
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Detection Range
31.25~2000pg/mL
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Target Name
TNF-β
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UNIProt ID
Q06332
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Rat TNF-β(Tumor Necrosis Factor Beta) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
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Gene target
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Gene symbol
TNF, TNFRSF1A, TNFRSF1B, LTBR
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Short name
TNF-β(Tumor Necrosis Factor Beta) EIA Kit
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Technique
EIA, eia
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Host
Rat
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Species
Rats
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Alternative name
Rat tumor necrosis factor-β(Tumor Necrosis Factor b) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
tumor
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Gene info
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Identity
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Gene
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Long gene name
tumor necrosis factor
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (TNF superfamily, member 2)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Tumor necrosis factor superfamily
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VEGA ID
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A cytologic technique for measuring the functional capacity of tumor stem cells by assaying their activity. It is used primarily for the in vitro testing of antineoplastic agents.
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Tree numbers
- E01.370.225.500.383.910
- E01.370.225.500.388.930
- E05.200.500.383.910
- E05.200.500.388.930
- E05.242.383.910
- E05.242.417.500
- E05.337.550.200.800
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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