Ramos [RA 1] Cell Line

• Catalog number
  EP-CL-0483
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  Ramos [RA 1]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  3 years
• Morphology
  Lymphoblast
• Growth_properties
  Suspension
• Doubling_time
  NA
• Background
  The cells are negative for Epstein-Barr virus. Both the parental line and the derivative cell line Ramos. 2G6. 4C10 have about 1500 IL-4 binding sites per cell. The cells are reported to secrete IgM(lambda light chain).
• Biosafety
  1
• Medium
  RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, forms tumors in nude mice.
• Receptor_expression
  Interleukin 4(IL-4); low affinity IgE(CD23)
• Antigen_expression
  CD23＋
• Gene_expression
  Immunoglobulin(Surface And Secreted)
• Applications
  Transfection.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Ramos   [RA   Line  
• Short name
  Ramos [RA 1] Line
• Species
  Homo sapiens, Human
• Alternative name
  Ramos [RA 1] cellular Line
• Tissue
  Peripheral blood

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