Raji Cell Line
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Catalog numberEP-CL-0189
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationRaji
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age11 years
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MorphologyLymphoblast
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Growth_propertiesSuspension
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Doubling_timeNA
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BackgroundThese cells are EBNA positive. The cells are partially resistant to poliovirus and vesicular stomatitis viruses. ATCC confirmed this line tested positive for the presence of Epstein Barr virus(EBV) viral DNA sequences via PCR.
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Biosafety2
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MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNA
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EffectsNA
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsTransfection.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Short nameRaji Line
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SpeciesHomo sapiens, Human
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Alternative nameRaji cellular Line
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TissueLymphoblast
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