PK-15 Cell Line

• Catalog number
  EP-CL-0187
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  PK-15
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  Adult
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The presence of a porcine papovavirus in PK-15 cells has been reported in cells obtained from multiple sources including the ATCC. The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that ATCC CCL-33 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses. The cell line harbors an endogenous C-type retrovirus. The cells are positive for porcine circovirus(PCV) antigens. The cells are positive for keratin by immunoperoxidase staining.
• Biosafety
  1
• Medium
  MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  PK-15   Line  
• Gene symbol
  PIRC67, PIRC66, PIRC71, PIRC75, PIRC68, PIRC62, PIRC72, PIRC70, PIRC77, PIRC76
• Short name
  PK-15 Line
• Species
  Sus scrofa, Pig
• Alternative name
  PK-15 cellular Line
• Tissue
  Kidney

Gene info

• Identity
  HGNC:37571
• Gene
  PIRC67
• Long gene name
  piwi-interacting RNA cluster 67
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313817
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37570
• Gene
  PIRC66
• Long gene name
  piwi-interacting RNA cluster 66
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313865
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37575
• Gene
  PIRC71
• Long gene name
  piwi-interacting RNA cluster 71
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313818
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37579
• Gene
  PIRC75
• Long gene name
  piwi-interacting RNA cluster 75
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313919
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37572
• Gene
  PIRC68
• Long gene name
  piwi-interacting RNA cluster 68
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313931
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37566
• Gene
  PIRC62
• Long gene name
  piwi-interacting RNA cluster 62
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313816
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37576
• Gene
  PIRC72
• Long gene name
  piwi-interacting RNA cluster 72
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313867
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37574
• Gene
  PIRC70
• Long gene name
  piwi-interacting RNA cluster 70
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313866
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37581
• Gene
  PIRC77
• Long gene name
  piwi-interacting RNA cluster 77
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313868
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37580
• Gene
  PIRC76
• Long gene name
  piwi-interacting RNA cluster 76
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313796
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

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