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Abbreviation
P815
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
NA
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Morphology
Rounded
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Growth_properties
Mixed, Adherent And Suspension
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Doubling_time
~18-22 hours
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Background
P815 cells phagocytose latex beads but not zymosan or BCG.They do not function in antibody dependent cell mediated cytotoxicity.Growth of the cells is not inhibited by dextran sulfate, LPS or PPD.Tested and found negative for ectromelia virus(mousepox).
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Biosafety
1
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Medium
DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
NA
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Effects
NA
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Receptor_expression
NA
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Antigen_expression
NA
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Gene_expression
Lysozyme
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Applications
Transfection host.
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
