OS-RC-2 Cell Line
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Catalog numberEP-CL-0177
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationOS-RC-2
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeNA
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundNA
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Biosafety1
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MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, forms tumors in nude mice.
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolRGN, INTS9, MID1, OS9, INTS11, MIR1289-2, MIR521-2, MIR509-2, MIR512-2, MIR7-2
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Short nameOS-RC-2 Line
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SpeciesHomo sapiens, Human
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Alternative nameOS-RC-2 cellular Line
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TissueKidney
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Gene info
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Identity
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Gene
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Long gene nameregucalcin
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Synonyms gene name
- regucalcin (senescence marker protein-30)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-02-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameintegrator complex subunit 9
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-07-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Integrator complex
- MBL fold containing DNA/RNA interacting subfamily
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemidline 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-12-12
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Pubmed identfication
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Classification
- Tripartite motif containing
- Fibronectin type III domain containing
- Ring finger proteins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameOS9 endoplasmic reticulum lectin
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Synonyms gene name
- osteosarcoma amplified 9, endoplasmic reticulum lectin
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2009-02-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MRH domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameintegrator complex subunit 11
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Synonyms gene
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Synonyms gene name
- cleavage and polyadenylation specific factor 3-like
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-05-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Integrator complex
- MicroRNA protein coding host genes
- MBL fold containing DNA/RNA interacting subfamily
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 521-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR515 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 512-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family