-
Abbreviation
OK
-
Leadtime
3-5 days
-
Shipping condition
Dry ice
-
Storage
Liquid nitrogen -196°C
-
Age
Adult
-
Morphology
Epithelial
-
Growth_properties
Adherent
-
Doubling_time
NA
-
Background
NA
-
Biosafety
1
-
Medium
MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
-
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
-
Ratio
1:2-1:4
-
Renewal
Every 2 to 3 days
-
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
Tumorigenic
NA
-
Effects
NA
-
Receptor_expression
NA
-
Antigen_expression
NA
-
Gene_expression
NA
-
Applications
This cell line is a suitable transfection host. This line was originally developed for use as a source of X chromosomes in a study of X inactivation, and was subsequently found to be an excellent cell culture model for the kidney proximal tubule epithelium. The cells display a variety of receptors in culture, and have been widely used as a model for studying those receptors.
-
Duration
NA
-
-
Description
For cells, cell lines and tissues in culture till half confluency.