-
Abbreviation
NIH/3T3
-
Leadtime
3-5 days
-
Shipping condition
Dry ice
-
Storage
Liquid nitrogen -196°C
-
Age
Embryo
-
Morphology
Fibroblast
-
Growth_properties
Adherent
-
Doubling_time
NA
-
Background
Tested and found negative for ectromelia virus(mousepox).
-
Biosafety
1
-
Medium
DMEM (PM150210)+10% CS+1% P/S (PB180120)
-
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
-
Ratio
1:2-1:4
-
Renewal
Every 2 to 3 days
-
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
Tumorigenic
NA
-
Effects
NA
-
Receptor_expression
NA
-
Antigen_expression
NA
-
Gene_expression
NA
-
Applications
This cell line is a suitable transfection host. The NIH/3T3 cell line is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies.
-
Duration
NA
-
-
Description
For cells, cell lines and tissues in culture till half confluency.
