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Abbreviation
NCI-H661
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
43 years
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Morphology
Epithelial
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Growth_properties
Adherent
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Doubling_time
NA
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Background
The line lacks ultrastructural and biochemical evidence of squamous differentiation or mucin production.The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities.The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.
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Biosafety
1
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Medium
RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
NA
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Effects
NA
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Receptor_expression
NA
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Antigen_expression
NA
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Gene_expression
NA
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Applications
NA
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.