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Abbreviation
NCI-H358
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
NA
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Morphology
Epithelial
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Growth_properties
Adherent
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Doubling_time
NA
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Background
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.SP-B and SP-C RNA was not expressed.They have a reported colony forming efficiency of 0.83% in soft agarose.
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Biosafety
1
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Medium
RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
Yes
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Effects
Yes, the cells produce tumors in athymic nude mice.
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Receptor_expression
NA
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Antigen_expression
NA
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Gene_expression
Lung Surfactant Associated Protein A(Sp-A), The Cells Expressed Protein And Rna Of Sp-A, The Major Lung Surfactant Associated Protein.
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Applications
This line was derived by A.F.Gazdar, H.K.Oie, J.D.Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
