MS1 Cell Line

  • Catalog number
    EP-CL-0162
  • Price
    Please ask
  • Size
    1×10⁶ cells/vial
  • Abbreviation
    MS1
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    NA
  • Morphology
    Endothelial
  • Growth_properties
    Adherent
  • Doubling_time
    NA
  • Background
    The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.The expression of high levels of the tissue inhibitor of bioreactive matrix metalloproteinase makes the behavior of this cell line more like that of normal macrophages from some commonly used strains of mice.
  • Biosafety
    2
  • Medium
    DMEM (PM150210)+5% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture_conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
  • Tumorigenic
    NA
  • Effects
    NA
  • Receptor_expression
    NA
  • Antigen_expression
    NA
  • Gene_expression
    NA
  • Applications
    This cell line is useful for the study of signal transduction of angiogenic factors. This cell line is the only line known that can give rise to benign hemangiomas.
  • Duration
    NA
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
    MS1   Line  
  • Short name
    MS1 Line
  • Species
    Mus musculus, Mouse
  • Alternative name
    MS1 cellular Line
  • Tissue
    Pancreas/islet of langerhans, endothelium
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