Mouse LEP (Leptin) CLIA Kit

• Catalog number
  E-CL-M0037-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Mouse
• Target Synonym
  LEPD, OB, OBS, Obesity Homolog
• Test principle
  This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse LEP . You can calculate the concentration of Mouse LEP in the samples by comparing the RLU value of the samples to the standard curve.
• Sensitivity
  9.38pg/mL
• Detection Range
  15.63~1000pg/mL
• Target Name
  LEP
• UNIProt ID
  NA
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Mouse LEP (Leptin) CLIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Gene
  Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  LEP   Leptin   CLIA   Kit  
• Gene symbol
  LEP
• Short name
  Mouse LEP (Leptin) CLIA Kit
• Technique
  Mouse, mouses
• Host
  mouse
• Species
  Mouse, Mouses
• Alternative name
  Mouse leptin (Leptin) CLIA reagent
• Alternative technique
  murine, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:6553
• Gene
  LEP
• Long gene name
  leptin
• Synonyms gene
  • OBS
  • OB
• Synonyms gene name
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
• Locus
  7q32.1
• Discovery year
  1993-01-26
• Entrez gene record
  3952
• Pubmed identfication
  • 1686014
  • 16932309
• Classification
  • Neuropeptides
• VEGA ID
  OTTHUMG00000157564

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