Mouse CD40L(Cluster of Differentiation 40 Ligand) EIA Kit
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Catalog number
E-EL-M0299-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Mouse
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Target Synonym
TNFSF5, CD40LG, CD154, HIGM1, IGM, IMD3, T-BAM, TRAP, Gp39
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD40L. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD40L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD40L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CD40L. You can calculate the concentration of Mouse CD40L in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
56.25 pg/mL
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Detection Range
93.75~6000pg/mL
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Target Name
CD40L
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UNIProt ID
P27548
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Mouse CD40L(Cluster of Differentiation 40 Ligand) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD40LG
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Short name
Mouse CD40L(Cluster of Differentiation 40 Ligand) EIA Kit
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Technique
Mouse, EIA, eia, mouses
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Host
mouse
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Species
Mouse, Mouses
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Alternative name
Mouse CD40L(Cluster on Differentiation 40 Ligand) EIA reagent
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Alternative technique
murine, kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
CD40 ligand
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Tumor necrosis factor superfamily
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VEGA ID
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Locus Specific Databases
Product images
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