MNNG/HOS Cl #5 [R-1059-D] Cell Line

  • Catalog number
    EP-CL-0492
  • Price
    Please ask
  • Size
    1×10⁶ cells/vial
  • Abbreviation
    MNNG/HOS Cl #5 [R-1059-D]
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    13 years
  • Morphology
    Epithelial-like
  • Growth_properties
    Adherent
  • Doubling_time
    NA
  • Background
    The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice.
  • Biosafety
    1
  • Medium
    MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture_conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
  • Tumorigenic
    Yes
  • Effects
    Yes, Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
  • Receptor_expression
    NA
  • Antigen_expression
    NA
  • Gene_expression
    NA
  • Applications
    NA
  • Duration
    NA
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
  • Gene symbol
    TBX5, SNAR-D, RRAS, DGCR11, DAD1P1, HNRNPR, CLDN4, MC1R
  • Short name
    MNNG/HOS Cl #5 [R-1059-D] Line
  • Species
    Homo sapiens, Human
  • Alternative name
    MNNG/HOS Cl #5 [R-1059-D] cellular Line
  • Tissue
    Bone
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    small NF90 (ILF3) associated RNA D
  • Synonyms gene name
    • small ILF3/NF90-associated RNA D
  • GenBank acession
  • Locus
  • Discovery year
    2008-07-03
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Small NF90 (ILF3) associated RNAs
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    DiGeorge syndrome critical region gene 11
  • Synonyms gene name
    • DiGeorge syndrome critical region gene 11
    • DiGeorge syndrome critical region gene 11 (non-protein coding)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2002-11-13
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Long non-coding RNAs with non-systematic symbols
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
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