ME-180 Cell Line

• Catalog number
  EP-CL-0155
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  ME-180
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  66 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  Desmosomal attachments were observed between cells in monolayer culture and cytoplasmic tonofilaments were noted. Contamination with Mycoplasma arginini was detected and eliminated in 1970. Growth of ME-180 cells is inhibited by tumor necrosis factor alpha(TNF alpha). The cells contain human papillomavirus(HPV) DNA with greater homology to HPV-39 than HPV-18.
• Biosafety
  2
• Medium
  McCoy's 5A (PM150710)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, in nude mice forms well differentiated epidermoid carcinoma(grade I).
• Receptor_expression
  NA
• Antigen_expression
  Blood Type A; Rh＋; HLA A1, A11, B5(＋/-), B40
• Gene_expression
  NA
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  ME-180   Line  
• Gene symbol
  LRRC7
• Short name
  ME-180 Line
• Species
  Homo sapiens, Human
• Alternative name
  ME-180 cellular Line
• Tissue
  Cervix, derived from metastatic site: omentum

Gene info

• Identity
  HGNC:18531
• Gene
  LRRC7
• Long gene name
  leucine rich repeat containing 7
• Synonyms
  • KIAA1365
  • densin-180
• Locus
  1p31.1
• Discovery year
  2004-11-10
• Entrez gene record
  57554
• Pubmed identfication
  • 12525888
• RefSeq identity
  • NM_020794
• Classification
  • PDZ domain containing
• VEGA ID
  OTTHUMG00000059194

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