MARC-145 [Marc-145; Marc 145; Marc145] Cell Line
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Catalog numberEP-CL-0566
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationMARC-145 [Marc-145; Marc 145; Marc145]
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeFoetus
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundNA
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Biosafety1
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MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNA
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EffectsNA
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolMIR145, RNVU1-8, VPS36, CCL7, OR1F1
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Short nameMARC-145 [Marc-145; Marc 145; Marc145] Line
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SpeciesCercopithecus aethiops
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Alternative nameMARC-145 [Marc-145; Marc 145; Marc145] cellular Line
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TissueEmbryonic kidney
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Gene info
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Identity
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Gene
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Long gene namemicroRNA 145
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNAs
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 8
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Synonyms
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GenBank acession
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Locus
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Discovery year2013-04-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namevacuolar protein sorting 36 homolog
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Synonyms gene
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Synonyms gene name
- chromosome 13 open reading frame 9
- vacuolar protein sorting 36 homolog (yeast)
- vacuolar protein sorting 36 homolog (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-01-24
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Entrez gene record
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Pubmed identfication
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Classification
- ESCRT-II
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 7
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Synonyms gene
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Synonyms gene name
- small inducible cytokine A7 (monocyte chemotactic protein 3)
- chemokine (C-C motif) ligand 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-11-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 1 subfamily F member 1
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 1, subfamily F, member 1
- olfactory receptor family 1 subfamily F member 1 (gene/pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-01-23
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Entrez gene record
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Pubmed identfication
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Classification
- Olfactory receptors, family 1
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VEGA ID