M-NFS-60 [NFS-60] Cell Line

Abbreviation

M-NFS-60 [NFS-60]
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

NA
Morphology

Lymphoblast
Growth_properties

Suspension
Doubling_time

NA
Background

The cells are responsive to both interleukin 3(interleukin-3, IL-3) and macrophage colony stimulating factor(M-CSF).The cells contain a truncated c-myb proto-oncogene caused by integration of a retrovirus.
Biosafety

1
Medium

RPMI-1640 (PM150110)+15ng/mL G-CSF+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

NA
Effects

NA
Receptor_expression

NA
Antigen_expression

NA
Gene_expression

NA
Applications

NA
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.

Gene target

M-NFS-60 [NFS-60] Line
Gene symbol

IGLV4-60, IGHVII-60-1, IGHV3-60, RCC2, OGFR, RNU6-60P, SPINK4, USP32, PPIL2, DYNC2LI1
Short name

M-NFS-60 [NFS-60] Line
Species

Mus musculus, Mouse
Alternative name

M-NFS-60 [NFS-60] cellular Line
Tissue

Bone marrow

Identity

HGNC:5686
Gene

IGHVII-60-1
Long gene name

immunoglobulin heavy variable (II)-60-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-60-1
- immunoglobulin heavy variable (II)-60-1 pseudogene
Synonyms
- IGHV(II)-60-1
GenBank acession
- AB019437
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28361
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000151951

Identity

HGNC:5614
Gene

IGHV3-60
Long gene name

immunoglobulin heavy variable 3-60 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable 3-60
- immunoglobulin heavy variable 3-60 pseudogene
GenBank acession
- M29813
Locus

14q32.33
Discovery year

2000-04-04
Entrez gene record

28417
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000151956

Identity

HGNC:9261
Gene

PPIL2
Long gene name

peptidylprolyl isomerase like 2
Synonyms gene name
- peptidylprolyl isomerase (cyclophilin)-like 2
Synonyms
- UBOX7
- CYC4
- Cyp-60
Synonyms name
- U-box domain containing 7
- peptidyl-prolyl cis-trans isomerase-like 2
Locus

22q11.21
Discovery year

1999-10-29
Entrez gene record

23759
Pubmed identfication
- 10591208
Classification
- Spliceosomal Bact complex
- U-box domain containing
- Cyclophilin peptidylprolyl isomerases
VEGA ID

OTTHUMG00000191763