LS 174T [LS174T] Cell Line

• Catalog number
  EP-CL-0145
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  LS 174T [LS174T]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  58 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  LS 174T is a variant of LS 180 that has been maintained by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen(CEA). Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles. They are negative for p53 antigen expression, but positive for mRNA expression. LS 174T cells stain positively for cytokeratins. The line is positive for expression of c-myc, N-myc, H-ras, N-ras, Myb, and fos oncogenes. K-ras and sis oncogene expression were not detected.
• Biosafety
  1
• Medium
  MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, in nude mice.
• Receptor_expression
  NA
• Antigen_expression
  serologically defined colon cancer antigen 3; Homo sapiens, expressed; HLA A2, B13, B50; Blood Type O
• Gene_expression
  Carcinoembryonic Antigen(Cea), Interleukin 10(Il-10), Interleukin 6(Il-6), Mucin; The Production Of Cea In The Atcc Seed Stock Was 1944Ng Per 10^6 Cells In 10 Days.
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  174T   [LS174T]   Line  
• Short name
  LS 174T [LS174T] Line
• Species
  Homo sapiens, Human
• Alternative name
  LS 174T [LS174T] cellular Line
• Tissue
  Colon

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