LS 174T [LS174T] Cell Line
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Catalog number
EP-CL-0145
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Price
Please ask
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Size
1×10⁶ cells/vial
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Abbreviation
LS 174T [LS174T]
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
58 years
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Morphology
Epithelial
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Growth_properties
Adherent
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Doubling_time
NA
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Background
LS 174T is a variant of LS 180 that has been maintained by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen(CEA). Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles. They are negative for p53 antigen expression, but positive for mRNA expression. LS 174T cells stain positively for cytokeratins. The line is positive for expression of c-myc, N-myc, H-ras, N-ras, Myb, and fos oncogenes. K-ras and sis oncogene expression were not detected.
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Biosafety
1
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Medium
MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
Yes
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Effects
Yes, in nude mice.
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Receptor_expression
NA
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Antigen_expression
serologically defined colon cancer antigen 3; Homo sapiens, expressed; HLA A2, B13, B50; Blood Type O
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Gene_expression
Carcinoembryonic Antigen(Cea), Interleukin 10(Il-10), Interleukin 6(Il-6), Mucin; The Production Of Cea In The Atcc Seed Stock Was 1944Ng Per 10^6 Cells In 10 Days.
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Applications
NA
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
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