-
Abbreviation
LoVo
-
Leadtime
3-5 days
-
Shipping condition
Dry ice
-
Storage
Liquid nitrogen -196°C
-
Age
56 years
-
Morphology
Epithelial
-
Growth_properties
Adherent
-
Doubling_time
NA
-
Background
The cells are negative for expression of CSAp(CSAp-) and colon antigen 3.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.N-myc and sis oncogene expression were not detected.Tumor specific nuclear matrix proteins CC-3 and CC-4 are expressed.
-
Biosafety
1
-
Medium
Ham's F-12K (PM150910)+10% FBS (164210-500)+1% P/S (PB180120)
-
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
-
Ratio
1:2-1:4
-
Renewal
Every 2 to 3 days
-
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
Tumorigenic
Yes
-
Effects
Yes, in nude mice.
-
Receptor_expression
NA
-
Antigen_expression
HLA A11, B15, B17, Cw1, Cw3; Blood Type B
-
Gene_expression
Carcinoembryonic Antigen(Cea) 908Ng/10^6 Cells/10Days. The Cells Are Negative For Expression Of Csap(Csap-) And Colon Antigen 3.
-
Applications
Transfection host.
-
Duration
NA
-
-
Description
For cells, cell lines and tissues in culture till half confluency.
