KG-1 Cell Line

Abbreviation

KG-1
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

59 years
Morphology

Myeloblast
Growth_properties

Suspension
Doubling_time

NA
Background

KG-1 myeloblasts can differentiate into macrophages in the presence of phorbol esters.They show a good response to colony stimulating factor(CSF).The line is EBNA negative(EBNA-).
Biosafety

1
Medium

IMDM (PM150510)+20% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:3.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

NA
Effects

NA
Receptor_expression

NA
Antigen_expression

HLA A30, A31, B35, Cw4
Gene_expression

Hla Dr
Applications

Transfection.
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.

Gene target

KG-1 Line
Gene symbol

IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1, PIRC1
Short name

KG-1 Line
Species

Homo sapiens, Human
Alternative name

KG-1 cellular Line
Tissue

Bone marrow

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081