K7M2 wt [K7M2-WT] Cell Line

Abbreviation

K7M2 wt [K7M2-WT]
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

Adult
Morphology

Macrophage
Growth_properties

Adherent
Doubling_time

NA
Background

NA
Biosafety

1
Medium

DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

Yes
Effects

Yes, forms tumors in BALB/c mice with spontaneous metastasis to the lungs in over 90% of mice inoculated.
Receptor_expression

Complement(C3), expressed; Fc receptor, IgG, high affinity I(Fcgr1), expressed
Antigen_expression

NA
Gene_expression

NA
Applications

Biological response. Transfection host
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.