K-562 [K562] Cell Line

• Catalog number
  EP-CL-0130
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  K-562 [K562]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  53 years
• Morphology
  Lymphoblast
• Growth_properties
  Suspension
• Doubling_time
  NA
• Background
  The cell population has been characterized as highly undifferentiated and of the granulocytic series. Studies conducted by Anderson, et al. , on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line. K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series. The effect of inducers on sublines derived from the original K-562 cell line have been reviewed by Koeffler and Golde. Karyological studies on various K-562 sublines have been classified into three groups(A,B,C) by Dimery, et al. The strain obtained by the ATCC most closely resembles the B population. Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined. The line is EBNA negative.
• Biosafety
  1
• Medium
  IMDM (PM150510)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
• Receptor_expression
  NA
• Antigen_expression
  CD7(25%)
• Gene_expression
  NA
• Applications
  Transfection.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  K-562   [K562]   Line  
• Gene symbol
  H4C13, MIR562, TUBA1B, RAP2A, GPC4, CPAMD8, H2BC10, KRAS
• Short name
  K-562 [K562] Line
• Species
  Homo sapiens, Human
• Alternative name
  K-562 [K562] cellular Line
• Tissue
  Bone marrow

Gene info

• Identity
  HGNC:4791
• Gene
  H4C13
• Long gene name
  H4 clustered histone 13
• Synonyms gene
  • H4FK
  • HIST1H4L
• Synonyms gene name
  • H4 histone family, member K
  • histone 1, H4l
  • histone cluster 1, H4l
  • histone cluster 1 H4 family member l
• Synonyms
  • H4.k
  • H4/k
• GenBank acession
  • X83548
• Locus
  6p22.1
• Discovery year
  1998-09-07
• Entrez gene record
  8368
• Pubmed identfication
  • 9031620
  • 9439656
  • 12408966
• RefSeq identity
  • NM_003546
• Classification
  • H4 histones
• VEGA ID
  OTTHUMG00000016211

Gene info

• Identity
  HGNC:32818
• Gene
  MIR562
• Long gene name
  microRNA 562
• Synonyms gene
  • MIRN562
• Synonyms
  • hsa-mir-562
• Locus
  2q37.1
• Discovery year
  2006-05-16
• Entrez gene record
  693147
• RefSeq identity
  • NR_030288
• Classification
  • MicroRNAs

Gene info

• Identity
  HGNC:18809
• Gene
  TUBA1B
• Long gene name
  tubulin alpha 1b
• Synonyms
  • K-ALPHA-1
• Synonyms name
  • tubulin, alpha, ubiquitous
• GenBank acession
  • AF081484
• Locus
  12q13.12
• Discovery year
  2007-02-12
• Entrez gene record
  10376
• Pubmed identfication
  • 12054644
  • 6646120
• RefSeq identity
  • NM_006082
• Classification
  • Tubulins
• VEGA ID
  OTTHUMG00000170410

Gene info

• Identity
  HGNC:9861
• Gene
  RAP2A
• Long gene name
  RAP2A, member of RAS oncogene family
• Synonyms gene
  • RAP2
• Synonyms
  • K-REV
• GenBank acession
  • AF205602
• Locus
  13q32.1
• Discovery year
  1989-06-30
• Entrez gene record
  5911
• Classification
  • RAS type GTPase family
• VEGA ID
  OTTHUMG00000017240

Gene info

• Identity
  HGNC:4452
• Gene
  GPC4
• Long gene name
  glypican 4
• Synonyms
  • K-glypican
• Synonyms name
  • glypican proteoglycan 4
• GenBank acession
  • AF030186
• Locus
  Xq26.2
• Discovery year
  1996-08-08
• Entrez gene record
  2239
• Pubmed identfication
  • 9787072
• RefSeq identity
  • NM_001448
• Classification
  • Glypicans
• VEGA ID
  OTTHUMG00000022434
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:23228
• Gene
  CPAMD8
• Long gene name
  C3 and PZP like alpha-2-macroglobulin domain containing 8
• Synonyms
  • KIAA1283
  • VIP
  • K-CAP
• GenBank acession
  • AY101765
• Locus
  19p13.11
• Discovery year
  2004-04-30
• Entrez gene record
  27151
• Pubmed identfication
  • 10574462
• RefSeq identity
  • NM_015692
• Classification
  • C3 and PZP like, alpha-2-macroglobulin domain containing
• VEGA ID
  OTTHUMG00000133549

Gene info

• Identity
  HGNC:4756
• Gene
  H2BC10
• Long gene name
  H2B clustered histone 10
• Synonyms gene
  • H2BFK
  • HIST1H2BI
• Synonyms gene name
  • H2B histone family, member K
  • histone 1, H2bi
  • histone cluster 1, H2bi
  • histone cluster 1 H2B family member i
• Synonyms
  • H2B/k
• GenBank acession
  • Z80782
• Locus
  6p22.2
• Discovery year
  1998-08-27
• Entrez gene record
  8346
• Pubmed identfication
  • 9119399
  • 12408966
• RefSeq identity
  • NM_003525
• Classification
  • H2B histones
• VEGA ID
  OTTHUMG00000014448

Gene info

• Identity
  HGNC:6407
• Gene
  KRAS
• Long gene name
  KRAS proto-oncogene, GTPase
• Synonyms gene
  • KRAS2
• Synonyms gene name
  • v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
  • v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
  • Kirsten rat sarcoma viral oncogene homolog
• Synonyms
  • KRAS1
  • K-Ras4B
• GenBank acession
  • BC010502
• Locus
  12p12.1
• Discovery year
  1988-06-02
• Entrez gene record
  3845
• RefSeq identity
  • NM_033360
• Classification
  • RAS type GTPase family
• VEGA ID
  OTTHUMG00000171193
• Locus Specific Databases
  • LRG_344

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