JeKo-1 Cell Line

• Catalog number
  EP-CL-0128
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  JeKo-1
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  78 years
• Morphology
  Lymphoblast
• Growth_properties
  Suspension
• Doubling_time
  NA
• Background
  The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. Ref JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype.
• Biosafety
  1
• Medium
  RPMI-1640 (PM150110)+20% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  CD3-, CD5＋, CD10＋, CD19＋
• Gene_expression
  Cd3-, Cd5＋, Cd10＋, Cd19＋
• Applications
  JeKo-1 cells are highly tumorigenic in SCID mice.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  JeKo-1   Line  
• Short name
  JeKo-1 Line
• Species
  Homo sapiens, Human
• Alternative name
  JeKo-1 cellular Line
• Tissue
  Peripheral blood, mantle cell lymphoma

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