J774A.1 Cell Line

• Catalog number
  EP-CL-0370
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  J774A.1
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  Adult
• Morphology
  Macrophage
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  J774A. 1 cells are active in antibody dependent phagocytosis. Their growth is inhibited by dextran sulfate, PPD and LPS. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta(Il1b) is synthesized continuously by this line.
• Biosafety
  1
• Medium
  DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Subcultures are prepared by scraping or blowing. For a 100mm disk,remove all but 10 mL culture medium (adjust amount accordingly for otherculture vessels). Dislodge cells from the flask substrate with a cell scraperor blowing directly with the pipettes; aspirate and add appropriatealiquots of the cell suspension into new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  Complement(C3), expressed; Fc receptor, IgG, high affinity I(Fcgr1), expressed
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  Biological response. Transfection host
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  J774A   Line  
• Short name
  J774A 1 Line
• Species
  Mus musculus, Mouse
• Alternative name
  J774A.1 cellular Line
• Tissue
  Ascites

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