HUV-EC-C [HUVEC] Cell Line

• Catalog number
  EP-CL-0122
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  HUV-EC-C [HUVEC]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  NA
• Morphology
  Endothelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  Endothelial Cell Growth Supplement(ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. The cells have a life expectancy of 50 to 60 population doublings.
• Biosafety
  1
• Medium
  Ham's F-12K (PM150910)+0.1mg/mL Heparin+0.03-0.05mg/mL ECGs+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  No
• Effects
  Yes, the cells did form colonies in semisolid medium; No, the cells were not tumorigenic in immunosuppressed mice.
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  Factor Ⅷ
• Applications
  Transfection.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  HUV-EC-C   [HUVEC]   Line  
• Gene symbol
  SOD3, SMC4, MYB, PCK1, SFTPC, VCX3B, MAF, RFX1, EGR4
• Short name
  HUV-EC-C [HUVEC] Line
• Species
  Homo sapiens, Human
• Alternative name
  HUV-EC-C [HUVEC] cellular Line
• Tissue
  Umbilical vein/vascular endothelium

Gene info

• Identity
  HGNC:11181
• Gene
  SOD3
• Long gene name
  superoxide dismutase 3
• Synonyms gene name
  • superoxide dismutase 3, extracellular
• Synonyms
  • EC-SOD
• Locus
  4p15.2
• Discovery year
  1988-05-08
• Entrez gene record
  6649
• Classification
  • Superoxide dismutases
• VEGA ID
  OTTHUMG00000128565

Gene info

• Identity
  HGNC:14013
• Gene
  SMC4
• Long gene name
  structural maintenance of chromosomes 4
• Synonyms gene
  • SMC4L1
• Synonyms gene name
  • SMC4 (structural maintenance of chromosomes 4, yeast)-like 1
  • SMC4 structural maintenance of chromosomes 4-like 1 (yeast)
• Synonyms
  • hCAP-C
  • CAP-C
• GenBank acession
  • AF092564
• Locus
  3q25.33
• Discovery year
  2001-02-22
• Entrez gene record
  10051
• Pubmed identfication
  • 9789013
  • 10319587
• Classification
  • Structural maintenance of chromosomes proteins
  • Condensin I subunits
  • Condensin II subunits
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000159006

Gene info

• Identity
  HGNC:7545
• Gene
  MYB
• Long gene name
  MYB proto-oncogene, transcription factor
• Synonyms gene name
  • v-myb avian myeloblastosis viral oncogene homolog
• Synonyms
  • c-myb
• Locus
  6q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  4602
• Pubmed identfication
  • 17599807
• Classification
  • Myb/SANT domain containing
• VEGA ID
  OTTHUMG00000015629
• Locus Specific Databases
  • The Globin Gene Server

Gene info

• Identity
  HGNC:8724
• Gene
  PCK1
• Long gene name
  phosphoenolpyruvate carboxykinase 1
• Synonyms gene name
  • phosphoenolpyruvate carboxykinase 1 (soluble)
• Synonyms
  • PEPCK-C
• Locus
  20q13.31
• Discovery year
  1990-09-10
• Entrez gene record
  5105
• Pubmed identfication
  • 1492743
• VEGA ID
  OTTHUMG00000032825

Gene info

• Identity
  HGNC:10802
• Gene
  SFTPC
• Long gene name
  surfactant protein C
• Synonyms gene
  • SFTP2
• Synonyms gene name
  • surfactant, pulmonary-associated protein C
• Synonyms
  • SP-C
  • PSP-C
  • SMDP2
  • BRICD6
• Synonyms name
  • BRICHOS domain containing 6
• Locus
  8p21.3
• Discovery year
  1988-05-11
• Entrez gene record
  6440
• Pubmed identfication
  • 1859376
  • 16709565
• RefSeq identity
  • NM_003018
• Classification
  • BRICHOS domain containing
• VEGA ID
  OTTHUMG00000163775

Gene info

• Identity
  HGNC:31838
• Gene
  VCX3B
• Long gene name
  variable charge X-linked 3B
• Synonyms
  • VCX-C
• Locus
  Xp22.31
• Discovery year
  2005-01-11
• Classification
  • Variable charge X/Y family
• VEGA ID
  OTTHUMG00000021106

Gene info

• Identity
  HGNC:6776
• Gene
  MAF
• Long gene name
  MAF bZIP transcription factor
• Synonyms gene name
  • v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog
• Synonyms
  • c-MAF
• Locus
  16q23.2
• Discovery year
  1991-08-01
• Entrez gene record
  4094
• Pubmed identfication
  • 14998484
• Classification
  • Basic leucine zipper proteins
• VEGA ID
  OTTHUMG00000137621

Gene info

• Identity
  HGNC:9982
• Gene
  RFX1
• Long gene name
  regulatory factor X1
• Synonyms gene name
  • regulatory factor X, 1 (influences HLA class II expression)
• Synonyms
  • EF-C
• Synonyms name
  • trans-acting regulatory factor 1
  • enhancer factor C
  • MHC class II regulatory factor RFX
• Locus
  19p13.12
• Discovery year
  1991-11-06
• Entrez gene record
  5989
• Pubmed identfication
  • 1505960
  • 8289803
• RefSeq identity
  • NM_002918
• Classification
  • Regulatory factor X family
• VEGA ID
  OTTHUMG00000181979

Gene info

• Identity
  HGNC:3241
• Gene
  EGR4
• Long gene name
  early growth response 4
• Synonyms
  • NGFI-C
  • PAT133
• Locus
  2p13.2
• Discovery year
  1992-07-27
• Entrez gene record
  1961
• Pubmed identfication
  • 1584812
• RefSeq identity
  • NM_001965
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000129774

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