Human LEP (Leptin) CLIA Kit

• Catalog number
  E-CL-H0112-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Human
• Target Synonym
  LEPD, OB, OBS, Obesity Homolog
• Test principle
  This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LEP . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEP , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LEP . You can calculate the concentration of Human LEP in the samples by comparing the RLU value of the samples to the standard curve.
• Sensitivity
  18.75pg/mL
• Detection Range
  31.25~2000pg/mL
• Target Name
  LEP
• UNIProt ID
  NA
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Human LEP (Leptin) CLIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Gene
  Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.

• Gene target
  LEP   Leptin   CLIA   Kit  
• Gene symbol
  LEP
• Short name
  LEP (Leptin) CLIA Kit
• Species
  Human, Humans
• Alternative name
  H. sapiens leptin (Leptin) CLIA reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:6553
• Gene
  LEP
• Long gene name
  leptin
• Synonyms gene
  • OBS
  • OB
• Synonyms gene name
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
• Locus
  7q32.1
• Discovery year
  1993-01-26
• Entrez gene record
  3952
• Pubmed identfication
  • 1686014
  • 16932309
• Classification
  • Neuropeptides
• VEGA ID
  OTTHUMG00000157564

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