Hi-five(BTI-TN-5B1-4) Cell Line

Abbreviation

Hi-five(BTI-TN-5B1-4)
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

NA
Morphology

Lymphoblast
Growth_properties

Mixed, Adherent And Suspension
Doubling_time

NA
Background

NA
Biosafety

1
Medium

TNM-FH (PM152010)+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air Temperature: 27℃
Tumorigenic

NA
Effects

NA
Receptor_expression

NA
Antigen_expression

NA
Gene_expression

NA
Applications

NA
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.

Gene target

Hi-five BTI-TN-5B1-4 Line
Gene symbol

CLEC3B, MT-TN, TNC, MIR1302-4, PIRC18, PIRC16, SNORD114-4, MIR941-4
Short name

Hi-five(BTI-TN-5B1-4) Line
Species

Spodoptera exigua
Alternative name

Hi-five(BTI-TN-5B1-4) cellular Line
Tissue

Insect

Identity

HGNC:11891
Gene

CLEC3B
Long gene name

C-type lectin domain family 3 member B
Synonyms gene
- TNA
Synonyms gene name
- tetranectin (plasminogen binding protein)
- C-type lectin domain family 3, member B
Synonyms
- TN
Locus

3p21.31
Discovery year

1993-08-26
Entrez gene record

7123
RefSeq identity
- NM_003278
Classification
- C-type lectin domain containing
VEGA ID

OTTHUMG00000133087

Identity

HGNC:5318
Gene

TNC
Long gene name

tenascin C
Synonyms gene
- HXB
- DFNA56
Synonyms gene name
- hexabrachion (tenascin C, cytotactin)
- deafness, autosomal dominant 56
Synonyms
- TN
- MGC167029
Synonyms name
- hexabrachion (tenascin)
Locus

9q33.1
Discovery year

1989-09-08
Entrez gene record

3371
Pubmed identfication
- 1704365
- 1707164
- 23936043
RefSeq identity
- NM_002160
Classification
- Tenascins
VEGA ID

OTTHUMG00000021010