HEL Cell Line

Abbreviation

HEL
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

NA
Morphology

Rounded
Growth_properties

Mixed, Adherent And Suspension
Doubling_time

~36 hours
Background

NA
Biosafety

1
Medium

RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

NA
Effects

NA
Receptor_expression

NA
Antigen_expression

NA
Gene_expression

NA
Applications

NA
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.

Identity

HGNC:3313
Gene

ELAVL2
Long gene name

ELAV like RNA binding protein 2
Synonyms gene name
- ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2
- ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
- ELAV like neuron-specific RNA binding protein 2
Synonyms
- HuB
- HEL-N1
Synonyms name
- Hu antigen B
GenBank acession
- BC030692
Locus

9p21.3
Discovery year

1996-11-15
Entrez gene record

1993
Pubmed identfication
- 8812435
RefSeq identity
- NM_004432
Classification
- RNA binding motif containing
VEGA ID

OTTHUMG00000019700