HCT-8 [HRT-18] Cell Line
-
Catalog numberEP-CL-0098
-
PricePlease ask
-
Size1×10⁶ cells/vial
-
-
AbbreviationHCT-8 [HRT-18]
-
Leadtime3-5 days
-
Shipping conditionDry ice
-
StorageLiquid nitrogen -196°C
-
Age67 years adult
-
MorphologyEpithelial
-
Growth_propertiesAdherent
-
Doubling_timeNA
-
BackgroundThe HCT-8 line is identical to the HRT-18 cell line.The cells are positive for keratin by immunoperoxidase staining.
-
Biosafety1
-
MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
-
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
-
Ratio1:2-1:4
-
RenewalEvery 2 to 3 days
-
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
TumorigenicYes
-
EffectsYes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
-
Receptor_expressionNA
-
Antigen_expressionNA
-
Gene_expressionCarcinoembryonic Antigen(Cea) 0.5Ng/10^6 Cells/10 Days; Alkaline Phosphatase. The Cells Are Positive For Keratin By Immunoperoxidase Staining.
-
ApplicationsNA
-
DurationNA
-
DescriptionFor cells, cell lines and tissues in culture till half confluency.
-
Gene target
-
Gene symbolSNORD114-18, PIRC96, SNORD116-18, IGLV2-18, IGHV1-18, SNORD114-8, ERVW-18, SNORD115-18, RNU6-8
-
Short nameHCT-8 [HRT-18] Line
-
SpeciesHomo sapiens, Human
-
Alternative nameHCT-8 [HRT-18] cellular Line
-
TissueColon
-
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 114-18
-
Synonyms
-
Locus
-
Discovery year2006-09-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 96
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 116-18
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable 2-18
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 1-18
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 114-8
-
Synonyms
-
Locus
-
Discovery year2006-09-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameendogenous retrovirus group W member 18
-
Synonyms gene name
- endogenous retrovirus group W, member 18
-
GenBank acession
-
Locus
-
Discovery year2013-10-21
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 115-18
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, U6 small nuclear 8
-
Synonyms
-
Locus
-
Discovery year2008-06-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nuclear RNAs