HCT-8 [HRT-18] Cell Line

  • Catalog number
    EP-CL-0098
  • Price
    Please ask
  • Size
    1×10⁶ cells/vial
  • Abbreviation
    HCT-8 [HRT-18]
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    67 years adult
  • Morphology
    Epithelial
  • Growth_properties
    Adherent
  • Doubling_time
    NA
  • Background
    The HCT-8 line is identical to the HRT-18 cell line.The cells are positive for keratin by immunoperoxidase staining.
  • Biosafety
    1
  • Medium
    RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture_conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
  • Tumorigenic
    Yes
  • Effects
    Yes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
  • Receptor_expression
    NA
  • Antigen_expression
    NA
  • Gene_expression
    Carcinoembryonic Antigen(Cea) 0.5Ng/10^6 Cells/10 Days; Alkaline Phosphatase. The Cells Are Positive For Keratin By Immunoperoxidase Staining.
  • Applications
    NA
  • Duration
    NA
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
    HCT-8   [HRT-18]   Line  
  • Gene symbol
    SNORD114-18, PIRC96, SNORD116-18, IGLV2-18, IGHV1-18, SNORD114-8, ERVW-18, SNORD115-18, RNU6-8
  • Short name
    HCT-8 [HRT-18] Line
  • Species
    Homo sapiens, Human
  • Alternative name
    HCT-8 [HRT-18] cellular Line
  • Tissue
    Colon
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 96
  • Locus
    18
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    endogenous retrovirus group W member 18
  • Synonyms gene name
    • endogenous retrovirus group W, member 18
  • GenBank acession
  • Locus
  • Discovery year
    2013-10-21
Gene info
Gene info
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