HCT-15 Cell Line
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Catalog numberEP-CL-0097
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationHCT-15
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeNA
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundHCT-15 cells are CSAp negative(CSAp-).The cells are positive for keratin by immunoperoxidase staining.
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Biosafety1
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MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionCarcinoembryonic Antigen(Cea) 5.4Ng/10^6 Cells/10 Days. The Cells Are Positive For Keratin By Immunoperoxidase Staining.
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ApplicationsTransfection host.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolPIRC67, PIRC66, PIRC71, PIRC75, PIRC68, PIRC62, PIRC72, PIRC70, PIRC77, PIRC76
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Short nameHCT-15 Line
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SpeciesHomo sapiens, Human
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Alternative nameHCT-15 cellular Line
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TissueColon
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 67
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 66
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 71
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 75
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
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Long gene namepiwi-interacting RNA cluster 68
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
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Long gene namepiwi-interacting RNA cluster 62
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Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 72
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Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 70
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 77
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Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 76
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters