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Abbreviation
DU 145
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
69 years
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Morphology
Epithelial
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Growth_properties
Adherent
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Doubling_time
NA
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Background
The line is not detectably hormone sensitive, is only weakly positive for acid phosphatase and isolated cells form colonies in soft agar. The cells do not express prostate antigen. Ultrastructural analyses of both the cell line and original tumor revealed microvilli, tonofilaments, desmosomes, any mitochondria, well developed Golgi and heterogenous lysosomes.
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Biosafety
1
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Medium
MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
Yes
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Effects
Yes, in nude mice forms adenocarcinoma(gradeⅡ) consistent with prostatic primary.
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Receptor_expression
NA
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Antigen_expression
Antigen expression: Blood Type O; Rh＋
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Gene_expression
NA
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Applications
Transfection host.
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
