DLD-1 Cell Line
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Catalog numberEP-CL-0074
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationDLD-1
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeAdult
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundThis cell line is one of four colorectal adenocarcinoma cell lines that have been identified as being derived from a single individual. DNA profiling studies [1,2] have shown that DLD-1, HCT-15, HCT-8 and HRT-18G share a single profile. DNA fingerprinting and cytogenetic analyses performed at ATCC and elsewhere show the line is similar to HCT-15 and suggest the two are of different clonal origin from the same individual. Their genetic origin has been confirmed by DNA fingerprinting; however, cytogenetic analysis has shown that they lack concurrent marker chromosomes or concurrent numerical changes. A culture of unknown passage submitted to the ATCC in 1979 was found to be contaminated with Mycoplasma hyorhinis. The cells were subsequently cured using a combination of antibiotics over a 12-week cultivation period. Following treatment, the cells were assayed by the Hoechst stain weekly and by the standard culture test periodically. During 11 consecutive months of cultivation in the absence of antibiotics, all of these tests were negative.
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Biosafety1
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MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionCarcinoembryonic Antigen(Cea) 0.5Ng/10^6 Cells/10 Days; Colon Antigen 3.
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ApplicationsTransfection host.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolDLD, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
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Short nameDLD-1 Line
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SpeciesHomo sapiens, Human
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Alternative nameDLD-1 cellular Line
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TissueColon
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Gene info
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Identity
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Gene
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Long gene namedihydrolipoamide dehydrogenase
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Synonyms gene
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Synonyms gene name
- dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-05-11
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Entrez gene record
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RefSeq identity
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Classification
- Pyruvate dehydrogenase complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 101-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR101 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 16-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR15/16 family