Daudi Cell Line

  • Catalog number
    EP-CL-0072
  • Price
    Please ask
  • Size
    1×10⁶ cells/vial
  • Abbreviation
    Daudi
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    16 years
  • Morphology
    Lymphoblast
  • Growth_properties
    Suspension
  • Doubling_time
    NA
  • Background
    The cells are negative for beta-2-microglobulin. They are positive for EBNA, VCA and Surface immunoglobulin(sIg+).
  • Biosafety
    2
  • Medium
    RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture_conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
  • Tumorigenic
    Yes, in nude mice; forms colonies in agarose.
  • Effects
    Yes, in agarose; Yes, in nude mice.
  • Receptor_expression
    Complement, Fc
  • Antigen_expression
    NA
  • Gene_expression
    NA
  • Applications
    Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis. The cell line is also a suitable transfection host.
  • Duration
    NA
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
    Daudi   Line  
  • Short name
    Daudi Line
  • Species
    Homo sapiens, Human
  • Alternative name
    Daudi cellular Line
  • Tissue
    Peripheral blood
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